Table 2.

	Qual domain (residues 14-67)		full STAR domain (residues 1-205)
QkI mutant	TM / °C	ΔTM / K	KD / nM	KD / KD(C35S)
wt	n.d.	n.d.	3.4 ± 0.2	0.7
C35S only	69	0	5.1 ± 0.3	1.0
Zipper
L18A	49	− 20	11.6 ± 0.6	2.3
Q20A	65	− 4	4.1 ± 0.3	0.8
L44A	37	− 32	118 ± 5	23
L45A	40	− 29	209 ± 9	41
I49A	38	−31	24 ± 1	4.7
V52A	60	−9	3.8 ± 0.2	0.7
Dimer interface and Zipper
Y17F	45	− 24	12.8 ± 0.7	2.5
L21A	< 15	< −57	340 ± 30	67
E48A	40	− 29	6.7 ± 0.4	1.3
E48G	< 15	< −57	22 ± 1	4.3
Dimer Interface
D24A	> 85	> +14	4.3 ± 0.2	0.8
L27A	67	− 2	3.5 ± 0.2	0.7
L31A	62	− 7	n.d.*	n.d.*
F34A	64	− 5	2.3 ± 0.1	0.5
I37A	67	− 2	4.4 ± 0.3	0.9
F38A	53	− 16	9.8 ± 0.6	1.9
H40A	58	− 11	10.8 ± 0.6	2.1
R43A	57	− 12	9.0 ± 0.5	1.8

Qkl-STAR L31A could not be expressed.