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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: J Neurochem. 2012 Sep 28;123(4):555–567. doi: 10.1111/j.1471-4159.2012.07955.x

Figure 3.

Figure 3

Changes in the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α in primary mouse microglial cell culture stimulated for 4 hr with LPS 6.25 ng/ml with and without 12 mM sodium acetate. Panel A shows representative images of the Western blots. Panels B, D and F show the optical densities of the pro-inflammatory cytokines pro-IL-1β, IL-6 and TNF-α, respectively, normalized to the loading control α-tubulin (n = 5). Panels C, E and G show the changes in the mRNA levels of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α, quantified by qrt-PCR and normalized to β-actin (n = 5). Bars represent means ± SD where statistical significance (a = compared to NaCl, and b = compared to LPS + NaCl) was set at p ≤ 0.05, as determined by a one way ANOVA followed by Tukey’s post-hoc test.