Changes in the phosphorylation state of MAPK p38, JNK and ERK1/2 in BV-2 microglia stimulated for 0.5, 1, 2 and 4 hr with LPS 6.25 ng/ml with and without 12 mM sodium acetate. Panel A shows representative images of the Western blots from the 4 hr experiment. Panels B, C and D show the optical densities of phosphorylated MAPK p38, JNK and ERK1/2 normalized to the loading controls MAPK p38, JNK and ERK1/2, respectively (n = 6). The data represent the means ± SD where statistical significance (* = compared to NaCl) was set at p ≤ 0.05, as determined by One Way ANOVA followed by Tukey’s post-hoc test.