Figure 3. UV-RNA cross-linking reveals exosome-RNA contacts.

Schematics of Exo10^44exo- (top), Exo10^6exo- (middle) and Exo11^44exo-/6exo- (bottom) shown on left. Subunits for which no cross-linking was observed are outlined, labeled and shown in white while subunits for which RNA-protein cross-links were detected are outlined, labeled and colored. Exosome subunits and exosomes were incubated with 5’ fluorescein AU-rich RNA and illuminated by UV to induce cross-linking. UV RNA-protein cross-linked products were separated by SDS-PAGE and gels were scanned to detect 5’-fluorescein RNA-protein adducts (middle), then stained with Sypro Ruby and scanned to visualize total protein (right). Subunit positions in respective gels indicated by labels and arrows. Molecular weight markers shown in left lane for the Sypro Ruby stained gel.