Figure 8. Effect of ethanol on ligand activation of ERK1/2.
CGNs were treated for 30 min with netrin-1, GDNF, L1-Fc, BDNF or 10−12M NAP, in the absence or presence of 25 mM ethanol. The ratio of pERK1/2 to ERK1/2 was normalized to control values in each experiment. Shown is the mean ± SEM normalized ratio following the indicated treatments. Representative immunoblots show phosphorylated ERK1/2 (top panels) and total ERK1/2 (bottom panels). * p < 0.05, ** p < 0.01, compared to control; # p < 0.05 between indicated groups (n= 4–10 independent experiments).