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. Author manuscript; available in PMC: 2013 Dec 14.
Published in final edited form as: J Immunol Methods. 2012 Sep 4;386(1-2):101–107. doi: 10.1016/j.jim.2012.08.014

Figure 1. FACS analysis of bone marrow cells with macrophage- and Foxp3-specific antibodies.

Figure 1

(A) CD11b+F4/80+ macrophage population was gated (gate R22), and “Foxp3 positive population” was determined (7.95%). The population was back gated in the SSC/FSC plot and distribution was showed in green dots. (B) Unstained, isotype, each individual antibody and antibody cocktail stained sample were analyzed. Autofluorescent population percentages were shown in the SSC/FL1(FITC) plots and FL1(FITC) histogram. Distribution of the population was shown in the SSC/FSC plots.