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Mean value±SEM of numerical density of neuron (neuron/mm3) in total hypothalamus of mice were fed with normal and high fat diet (HFD) for short and long-term. Group: 1, short-term control; 2, long-term control; 3, short-term HFD; 4, long-term HFD. *Significant difference with short-term control group (P<0.005). †Significant difference with long-term control group (P<0.001).
