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. Author manuscript; available in PMC: 2013 Nov 10.
Published in final edited form as: J Mol Biol. 2012 Aug 9;423(5):664–676. doi: 10.1016/j.jmb.2012.07.027

Figure 5. Two RNA Binding States of Rho.

Figure 5

(a) Diagram depicting the two Rho binding states consistent with the observed rips. Left: RNA bound to six Rho primary sites in its “open” configuration. Right: RNA bound to six primary sites plus the central secondary site in the “closed” configuration.

(b) Global histogram (grey bars) of the sizes of RNA-release rips observed in the presence of ATP or AMP-PNP for all templates studied (N =570). Solid line: Fit to sum of two Gaussians, with peaks at 28 ±1 nm and 45 ±1 nm (mean ± S.E.M.). Data from populations centered at 56 nm and 84 nm(Fig. 3) were included in the global dataset by dividing the measured values by 2 or 3, respectively (see text).