Figure 9.

Memory Phenotype. Homologous prime boost regimens favor a T_cm cell phenotype in the peripheral blood of Ad5 immmune mice. Memory phenotype was defined as percent of CSP (NYD) tetramer positive cells that are T_em cells (CD62L^lo CD127⁺) and percentage that are T_cm cells (CD62L⁺ CD127⁺) as opposed to percent of T_em and T_cm cells that are Tet⁺. PBMCs were collected on day 14 post final injection and stained according to the above defined memory phenpotype. Example of gating appears above the graphs (A). Percentage of T_cm Cells was significantly higher PBMCs from homologously boosted animals in Ad5 immune mice (B). There was no significant difference in the percentage of T_em cells present between any of the groups (C). Bars represent ± standard error. Statistical analysis was completed using One Way ANOVA with Student-Newman-Keuls post-hoc Test.