Table 1.
Characteristics of 50 EST-SSR primers developed in L. luteus. Shown for each primer pair are the library specificity, repeat motif, forward and reverse sequence, allele range size (bp), number of alleles, amplification in other Lupin species, and annotation
| Marker name | Library | Repeat motif | Forward primer (5′-3′) | Reverse primer (5′-3′) | Size (bp) | No of alleles | Amplification | Annotation |
|---|---|---|---|---|---|---|---|---|
| l1l2itg33000 |
L1 |
(ACA)7 |
CACGTCAGTCCTTGCACCTA |
GCACAGCAACAACAACACAA |
129-132 |
2 |
L.hispanicus |
|
| l1l2itg51784 |
L1 |
(TA)8 |
CATCCTTCAAAAACCATTTCAA |
AATGTTGATGAACGCGTGTG |
274-280 |
3 |
|
|
| l1l2itg52347 |
L1 |
(AT)8 |
CTCATGTTTCTTGGGTGGAAA |
CAATCATGTCTAAACCGGGAA |
209-215 |
4 |
|
|
| l1l2itg50343 |
L1 |
(AT)10 |
ATATTAGCGGCCATGCTGTT |
TGTTCATGTTGGTTGCAAGA |
235-239 |
3 |
|
|
| l1l2itg20858 |
L1 |
(AAC)12 |
ACCCCACTTCTCCCAACTCT |
TCCATGAATGAAATGGGGTT |
229-238 |
3 |
L.hispanicus |
Pollen-specific protein SF3 |
| l1l2itg20038 |
L1 |
(TA)9 |
TTCAGAAACAAAGGGGTTGC |
TCCAGAAATTCTTCTACATCCCA |
179-183 |
3 |
|
|
| l1l2itg52625 |
L1 |
(TCA)12 |
CTGGTCTTCTGTCGACTCCA |
GACCAAGAAGTCAAGCTCGG |
109-124 |
4 |
|
|
| l1l2itg37631 |
L1 |
(CT)12 |
TAAAGTGCCACCAACAAGCA |
TTGTGTTGGTTGTGTGTAGAGAGA |
133-155 |
6 |
|
|
| l1l2itg27097 |
L1 |
(AAT)7 |
TTCAACTACCGGTTGAACCAC |
GCCCAGAATTAGGGTGCTTT |
206-209 |
2 |
|
|
| l1l2itg22424 |
L1 |
(GAA)7 |
AAACGACCAACCGCATAAAG |
GATGCGTGAAACTGCAAAGA |
240-249 |
3 |
L.hispanicus |
N-acetylglutamate synthase |
| l1l2itg29703 |
L1 |
(GA)8 |
ACCTTTGCGCCAAGATACAC |
ATTGTGACGGTTTCACTCCC |
213-219 |
4 |
|
|
| l1l2itg28437 |
L1 |
(TA)9 |
GGGCACATTTGACTCTTTCG |
TCCGTGCAATGTCAATATCAA |
260-268 |
4 |
L.hispanicus |
|
| l1l2itg36804 |
L1 |
(ATA)12 |
CACATGAGAAGCAGCAATGAA |
ATGCGGTGGAGTGGAAGTAA |
254-260 |
2 |
L.hispanicus |
|
| l1l2itg21177 |
L1, L2 |
(CAT)8 |
CCTTGAGGCCAATAAATGGA |
TTAAGGAAGCTAGGGCCACA |
217-226 |
3 |
L.hispanicus |
Delta-8 sphingolipid desaturase |
| l1l2itg39645 |
L1 |
(ATT)10 |
AATCATGGCCTTTTTGCTTG |
CGTCTTGCTCTGGTTCTTCC |
148-169 |
5 |
|
|
| l1l2itg35309 |
L1 |
(TA)8 |
TTCATGGCAAGAAAAACATCT |
AATCATCCATGCCATTTAACA |
271-281 |
4 |
|
|
| l1l2itg56943 |
L1 |
(GA)8 |
GAGGCCCAAAAACAGAAACA |
CCATTTGCGTTCGGTTCTAT |
270-272 |
2 |
L.hispanicus, L.mutabilis |
|
| l1l2itg31693 |
L1 |
(TAT)8 |
AGGGGCAAAGCTCAAAGACT |
CATTCACATTTTATCCTCATTGACTC |
196-217 |
4 |
L.hispanicus |
|
| l1l2itg10347 |
L1 |
(AT)8 |
TGTGGTAAATGCAGGCTCAG |
ATGCAACGGGAACCATAGTC |
184-186 |
2 |
L.hispanicus |
|
| l1l2itg14618 |
L1 |
(CAT)7 |
TTCCTCATCTCCCACACCTC |
AGCTTCTGCTTGTAATCGGC |
237-252 |
4 |
|
|
| l1l2itg20466 |
L1, L2 |
(TA)9 |
GTAATCATTCATGTATAATTGTAACACTC |
CAATTCATTATCTGTATTATTACCCC |
180-186 |
3 |
|
Cytochrome B561 |
| l1l2itg53474 |
L1, L2 |
(GA)10 |
CTGAAGTGAGGTTCGGGAAG |
TCAATCACACATGCTTGTTCC |
230-234 |
3 |
|
Cullin-1 |
| l1l2itg51894 |
L1 |
(AT)10 |
TGACTTTGATTGTTTAGCTTACAGG |
TGAATGTCAAATGCAATATTAAGGA |
247-263 |
3 |
L.hispanicus |
|
| l1l2itg24819 |
L1 |
(AT)8 |
CATTCATTCTCTAATCTTTTGTGTCA |
TAAAGCTTGTCTCTTGCCCG |
219-244 |
5 |
L.hispanicus |
|
| l1l2itg55310 |
L1 |
(TA)9 |
ACCAAAAGGGTGGGTGAAAT |
CCTAACATTTGAACATATTTAAAACAA |
277-283 |
4 |
|
|
| l1l2itg14694 |
L1, L2 |
(TA)8 |
AAGTAGGAAGATCGAATATGAACG |
GGGAAAATATCGAGGTTTTCATC |
268-278 |
3 |
L.hispanicus, L.mutabilis |
RNA-binding protein |
| l1l2itg35641 |
L1 |
(AT)8 |
AGTTGCAATTCAACAACGCA |
CATGCTCTATGGCAAGTGCT |
247-251 |
3 |
|
|
| l1l2itg38340 |
L1 |
(TAT)7 |
AGCTCCACTTTTAGAATTGCG |
TCTATTGTTACATGCACATTATCCC |
164-173 |
4 |
L.hispanicus |
|
| l1l2itg26293 |
L2 |
(TCCGAA)15 |
CCTGCAGTGGTAGAACCTGG |
GAAGCAAGGTCCACAGAAGG |
123-183 |
6 |
|
18S ribosomal RNA gene |
| l1l2itg42878 |
L2 |
(CATTCC)11 |
CAACTCTTGTTTGCAGACCG |
GCTACCCTTTCGGGACTAGC |
217-235 |
4 |
L.hispanicus, L.mutabilis |
|
| l1l2itg13749 |
L2 |
(TTCCGC)8 |
TTTTTACTCGACTCGCTCCC |
CCAGTCGATTTAGCAGTCGC |
207-261 |
7 |
L.hispanicus, L.mutabilis |
|
| l1l2itg32760 |
L1, L2 |
(CGGAAT)14 |
TCATAATGAATTAAATTAACCCCC |
TCCCTGACTCTGTCTTTGGG |
146-284 |
14 |
L.hispanicus |
|
| l1l2itg00675 |
L2 |
(TCT)8(TCG)5 |
AGAGAGATCCTCTTTGACGCC |
GTGGTTAGCGAGAACCATCG |
187-199 |
4 |
|
BSD domain-containing protein |
| l1l2itg45631 |
L2 |
(ATC)10 |
AAACCGAATTGTGGATCAGC |
GGGGACTCTGGAAAATCAGG |
146-155 |
3 |
L.hispanicus, L.mutabilis |
Alphavirus core protein family |
| l1l2itg20349 |
L2 |
(AAC)7 |
ACTAAGGGAAAGGGATTCGG |
CCAGGCAAGAACAAAAGAGG |
186-189 |
2 |
L.hispanicus, L.mutabilis |
LPA2 (low psii accumulation2) |
| l1l2itg41827 |
L2 |
(TTG)7 |
TTGAGTCATATCACCATAGCGG |
CAACCACAAATGGAAAACCC |
242-245 |
2 |
L.hispanicus, L.mutabilis |
Lipase class 3 family protein |
| l1l2itg47916 |
L2 |
(TCT)9 |
GGTGGGTGAAAATGAAATGG |
TAACCAAAATGGTTCGTCGG |
241-247 |
2 |
L.hispanicus, L.mutabilis |
|
| l1l2itg42002 |
L2 |
(AAC)8 |
CTTGCAGGGTCTTCTTACAGC |
GGGGTTGTTTTTGGTGTCC |
243-246 |
2 |
L.hispanicus |
|
| l1l2itg54849 |
L2 |
(ACA)7 |
TTCTCCAATGATGAAATGCC |
TTCACGGCTAAATACCAAGC |
177-183 |
2 |
L.hispanicus |
Microtubule-associated protein |
| l1l2itg13638 |
L2 |
(TGT)9 |
CCATGGTCATCATTAACCCC |
CGAGTCGAGTTCGTTTACCC |
188-200 |
5 |
L.hispanicus, L.mutabilis |
f-box family protein |
| l1l2itg26640 |
L2 |
(AG)7 |
GGTCTGTTGGAGAAGGCTACC |
CCACCAATGGGTAGACATACG |
203-209 |
3 |
L.hispanicus |
Small nuclear ribonucleoprotein |
| l1l2itg29887 |
L2 |
(GCT)10 |
CCCATCTGAAAGACTTACGGC |
TCCCTTTTCATCCAGAGAGG |
243-249 |
2 |
L.hispanicus |
Ser/thr-protein kinase AFC2 |
| l1l2itg50945 |
L2 |
(CCA)6(ACA)7 |
CCAGAACAAGGAGAAGGTTCC |
TTCTTCTTCCTCGCAGGC |
198-204 |
3 |
L.hispanicus |
Zinc finger, Transcription factor |
| l1l2itg44905 |
L2 |
(CTT)9 |
AAATCACAGAGCCAAGGAGG |
TCAGCTTATTTTGTTTCCAAGC |
356-362 |
3 |
L.hispanicus, L.mutabilis |
Transcription factor |
| l1l2itg09113 |
L2 |
(AT)8 |
CATGACCCAATCTCAAACCC |
GCATCTGGATCTGCTTAATTGG |
341-343 |
2 |
L.hispanicus |
|
| l1l2itg03938 |
L2 |
(CCGATT)9 |
CATGTGGGAAGACCAGAAGC |
ACTACGCGCTGCTAATGTCC |
212-290 |
7 |
L.hispanicus, L.mutabilis |
Polygalacturonase |
| l1l2itg32421 |
L2 |
(AATCGG)8 |
AGAGAAGTAGGCATGGTGGC |
GATCGGCCTATTCACTCAGC |
221-293 |
5 |
L.hispanicus, L.mutabilis |
|
| l1l2itg29217 |
L1, L2 |
(AT)7 |
ACACTCTCAAGGAAAAGGGC |
CCATTTAACCGATAATGCTTGG |
340-344 |
2 |
L.hispanicus |
Lactoylglutathione lyase |
| l1l2itg27515 |
L2 |
(TTC)17 |
CATGCGTCCAATCTATCACC |
AGTGGGAAACAAGGAAGTGG |
182-221 |
8 |
L.hispanicus, L.mutabilis |
PPR-containing protein |
| l1l2itg41211 | L2 | (GAA)11 | TCCTCCTGCTTCAGAACG | AAATCCACGTCATCAATCCG | 209-230 | 6 | L.hispanicus, L.mutabilis |