Table 2.
Validation of microarray results by real-time quantitative PCR
|
Gene expression fold change after ETEC infection in each group | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
(Inf./Non.) | |||||||||||
|
CF4abvscontrol* |
CF4acvscontrol |
CF18acvscontrol |
|||||||||
| Genes | Probe Name in Micro-array | Micro-array | Q-PCR# | Genes | Probe Name in Micro-array | Micro-array | Q-PCR | Genes | Probe Name in Micro-array | Micro-array | Q-PCR |
|
IL8 |
A_72_P232367 |
5.03** |
4.09**(G) 5.50*(A) |
MUC20 |
A_72_P440416 |
−1.33* |
−1.82**(G) −1.23(A) |
FUT1 |
A_72_P146581 |
1.13 |
1.00(G) 1.04(A) |
|
SDC2 |
A_72_P098981 |
−2.57** |
−2.84**(G) −2.06(A) |
ANK3 |
A_72_P628816 |
−2.90** |
−4.40**(G) −2.99(A) |
CXCL2 |
A_72_P146411 |
2.31* |
2.31*(G) 2.29*(A) |
|
UPK2 |
A_72_P441773 |
4.27** |
3.69**(G) 5.21*(A) |
IRG6 |
A_72_P443124 |
4.86** |
4.67*(G) 6.75**(A) |
IRG6 |
A_72_P443124 |
2.12* |
1.62(G) 1.64*(A) |
|
MUC4 |
A_72_P140196 |
−1.46 |
−2.04(G) −1.28(A) |
LOC10015251 |
A_72_P527727 |
−1.25 |
−1.91(G) −1.42(A) |
|
|
|
|
| MUC13 | A_72_P442057 | −3.01** | −5.66**(G) −3.46**(A) | ||||||||
* CF4ab, IPEC-J2 cells infected with F4ab ETEC; CF4ac, IPEC-J2 cells infected with F4ac ETEC ; CF18ac, IPEC-J2 cells infected with F18ac ETEC ; Control: the non-infected IPEC-J2 cells. # G: gene expression level related to GAPDH; A: gene expression level related to ACTB. The numbers here represent the fold change of gene expression after ETEC infection. The numbers equal to 1.00 means the expression level doesn’t change after ETEC infection. The numbers > 1.00 means the expression level is increased after ETEC infection while numbers < 1.00 indicate that the expression level is reduced after ETEC infection. The numbers with “*” are statistically significant (P < 0.05), and which with“**” are statistically very significant (P < 0.01).