Skip to main content
. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: Dev Biol. 2012 Aug 30;371(2):246–255. doi: 10.1016/j.ydbio.2012.08.016

Figure 4. Beta-catenin knockout grafts do not show prostatic differentiation.

Figure 4

Renal grafts from E15.5 CreERT1 Ctnnb1fl/fl UGSs grown after 48 hour in vitro culture with vehicle (A–G) or tamoxifen (H–N) before 4 weeks growth as subcapsular renal grafts. Vehicle treated grafts show normal prostate gland formation (B, C), and express beta-catenin (D), Nkx3.1 (E), Hoxb13 (F), and AR (G). Beta-catenin deletion resulted in smaller grafts compared to controls (A, H), and H&E staining demonstrates disorganized grafts without recognizable prostatic differentiation (I, J). IHC analysis showed loss of beta-catenin (K), and Nkx3.1 (L). Hoxb13 expression was absent in the majority of tissue (M), with expression limited to small foci of cells with periurethral gland differentiation (inset). Grafted tissue retains AR expression (N). Scale bars 500 micrometers in B, I. All others 50 micrometers.