Table 3.
Uniformity analysis for preamplification
| Target | Pre | Cq | ΔΔCq | ΔCq | Ampl | ΔCq 250 ng/ΔCq 2.5 ng |
|---|---|---|---|---|---|---|
|
Lung 250 ng cDNA | ||||||
| S1PR2 | + | 13.518 | 0.638 | 11.712 | 0.948 | 1.013 |
| S1PR2 | − | 25.230 | ||||
| S1PR5 | + | 16.280 | 0.224 | 12.126 | 0.982 | 0.911 |
| S1PR5 | − | 28.406 | ||||
| Arbp | + | 9.098 | −0.388 | 12.738 | 1.031 | 1.038 |
| Arbp | − | 21.837 | ||||
| HPRT | + | 12.833 | 0 | 12.350 | 1.0 | 1.0 |
| HPRT | − | 25.183 | ||||
|
Lung 2.5 ng cDNA | |||||
|---|---|---|---|---|---|
| Target | Pre | Cq | ΔΔCq | ΔCq | Ampl |
| S1PR2 | + | 21.468 | 0.770 | 11.564 | 0.938 |
| S1PR2 | − | 33.032 | |||
| S1PR5 | + | 23.348 | −0.974 | 13.309 | 1.079 |
| S1PR5 | − | 36.656 | |||
| Arbp | + | 16.635 | 0.060 | 12.274 | 0.995 |
| Arbp | − | 28.909 | |||
| HPRT | + | 20.244 | 0 | 12.335 | 1.0 |
| HPRT | − | 32.579 | |||
Pre, preamplification; Cq, quantitation cycle; GOI, gene of interest; ΔΔCq, ΔCqpreamp− ΔCqnoampwithin a gene target where ΔCqpreamp= CqGOIpreamp− CqHPRTpreampand ΔCqnoamp= CqGOInoamp− CqHPRTnoamp; ΔCq = Cqnoamp− Cqpreampwithin a gene target; Ampl: amplification within a gene target = ΔCqGOI− ΔCqHPRT.