Table 3.
Cardiac mitochondrial proteins with increased abundance in aging and E2 deficiency
| Unused Score | Total Score | % Coverage | Accession # | Protein Identified | Quantitation Ratio Aged/Adult | P Value | Error Factor | Quantitation Ratio Adult OVX/Adult | P Value | Error Factor | Quantiation Ratio Aged OVX/Adult | P Value | Error Factor | PANTHER Biological Process |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 15.3 | 15.3 | 50.9 | gi 8394331 | superoxide dismutase 2, mitochondrial (SOD2) | 1.22 | 0.0038 | 1.13 | 1.02 | 0.43 | 1.21 | 1.38 | 0.0031 | 1.21 | antioxidation and free radical removal |
| 4.0 | 4.0 | 19.7 | gi 38511566 | 3-hydroxy-3-methylglutaryl-Coenzyme A lyase | 1.07 | 0.6542 | 4.07 | 1.31 | 0.47 | 18.95 | 1.47 | 0.0185 | 1.15 | metabolism: Acyl-CoA metabolism |
| 32.4 | 32.7 | 58.3 | gi 6978431 | acyl-Coenzyme A dehydrogenase, long-chain | 1.20 | 0.0142 | 1.12 | 1.05 | 0.29 | 1.09 | 1.25 | 0.0003 | 1.09 | metabolism: Acyl-CoA metabolism; electron transport |
| 23.7 | 23.7 | 51.3 | gi 8392833 | acyl-Coenzyme A dehydrogenase, medium chain | 1.19 | 0.0084 | 1.12 | 1.06 | 0.34 | 1.10 | 1.19 | 0.0066 | 1.09 | metabolism: Acyl-CoA metabolism; electron transport |
| 20.7 | 20.7 | 51.0 | gi 48734846 | acyl-Coenzyme A dehydrogenase, short chain | 0.83 | 0.0500 | 1.17 | 1.01 | 0.50 | 1.20 | 1.22 | 0.0149 | 1.13 | metabolism: Acyl-CoA metabolism; electron transport |
| 30.5 | 30.5 | 60.2 | gi 6980972 | glutamate oxaloacetate transaminase 2 | 1.10 | 0.0370 | 1.09 | 1.06 | 0.50 | 1.16 | 1.19 | 0.0287 | 1.09 | metabolism: amino acid metabolism |
| 28.3 | 28.3 | 51.0 | gi 81885266 | dihydrolipoyl dehydrogenase, mitochondrial precursor (dihydrolipoamide dehydrogenase) | 1.20 | 0.0146 | 1.12 | 1.07 | 0.31 | 1.13 | 1.30 | 0.0001 | 1.10 | metabolism: electron transport |
| 30.8 | 30.8 | 44.3 | gi 55605 | aldehyde dehydrogenase preprotein | 1.25 | 0.0127 | 1.12 | 1.05 | 0.35 | 1.11 | 1.26 | 0.0020 | 1.13 | metabolism: other metabolism |
| 14.5 | 14.5 | 95.7 | gi 6981260 | NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5 | 1.08 | 0.4442 | 1.23 | 1.10 | 0.32 | 1.19 | 1.14 | 0.0094 | 1.10 | metabolism: oxidative phosphorylation |
| 13.3 | 13.3 | 36.5 | gi 157820787 | NADH dehydrogenase (ubiquinone) 1, alpha/beta subcomplex, 1 | 1.11 | 0.0916 | 1.13 | 1.32 | 0.42 | 1.26 | 1.25 | 0.0105 | 1.16 | metabolism: oxidative phosphorylation |
| 14.1 | 14.1 | 45.8 | gi 57164133 | NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2 | 1.07 | 0.2319 | 1.12 | 1.13 | 0.24 | 1.16 | 1.15 | 0.0095 | 1.10 | metabolism: oxidative phosphorylation |
| 33.8 | 33.8 | 90.5 | gi 6978725 | cytochrome c, somatic | 1.20 | 0.0066 | 1.14 | 1.20 | 0.32 | 1.17 | 1.12 | 0.0617 | 1.13 | metabolism: oxidative phosphorylation; apoptotic processes |
| 58.2 | 58.2 | 43.5 | gi 81883712 | 2-oxoglutarate dehydrogenase E1 component, mitochondrial precursor (Alpha-ketoglutarate dehydrogenase) | 1.03 | 0.5217 | 1.09 | 1.08 | 0.16 | 1.09 | 1.22 | 0.0004 | 1.09 | metabolism: tricarboxylic acid pathway |
| 78.6 | 78.6 | 70.4 | gi 60391194 | aconitate hydratase, mitochondrial precursor (Citrate hydro-lyase) (Aconitase) | 1.09 | 0.1683 | 1.10 | 0.99 | 0.47 | 1.08 | 1.09 | 0.0146 | 1.07 | metabolism: tricarboxylic acid pathway |
| 31.4 | 31.4 | 48.3 | gi 81902084 | citrate synthase, mitochondrial precursor | 1.05 | 0.0722 | 1.11 | 1.05 | 0.30 | 1.10 | 1.18 | 0.0024 | 1.09 | metabolism: tricarboxylic acid pathway |
| 24.1 | 24.1 | 50.0 | gi 149025179 | dihydrolipoamide S-succinyltransferase (E2 component of 2-oxo-glutarate complex), isoform CRA_a | 1.30 | 0.0108 | 1.19 | 0.99 | 0.65 | 1.26 | 1.42 | 0.0167 | 1.29 | metabolism: tricarboxyli c acid pathway |
| 22.0 | 22.0 | 49.7 | gi 68565369 | isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial precursor (Isocitric dehydrogenase) | 1.06 | 0.17 | 1.16 | 1.00 | 0.57 | 1.14 | 1.15 | 0.0048 | 1.10 | metabolism: tricarboxylic acid pathway |
| 47.7 | 47.8 | 76.8 | gi 42476181 | malate dehydrogenase, mitochondrial | 1.18 | 0.0101 | 1.11 | 1.04 | 0.38 | 1.08 | 1.21 | 0.0025 | 1.08 | metabolism: tricarboxylic acid pathway |
| 27.4 | 27.4 | 85.34 | gi 205495 | cardiac myosin light chain 2 | 1.84 | 0.0300 | 1.24 | 1.05 | 0.20 | 1.09 | 1.40 | 0.0400 | 1.26 | muscle contraction |
| 50.2 | 50.2 | 65.9 | gi 8392836 | acetyl-coenzyme A acetyltransferase 1 | 1.19 | 0.0084 | 1.12 | 1.06 | 0.34 | 1.10 | 1.19 | 0.0066 | 1.09 | protein acetylation |
| 56.5 | 56.6 | 69.8 | gi 56383 | heat shock protein (Hsp60) precursor | 1.12 | 0.0042 | 1.08 | 1.03 | 0.23 | 1.10 | 1.16 | 0.0043 | 1.10 | protein folding; protein complex assembly |
| 3.9 | 4.0 | 20.6 | gi 71051777 | heat shock protein 8 | 1.07 | 0.3258 | 1.24 | 1.30 | 0.12 | 1.54 | 1.34 | 0.0479 | 1.41 | protein folding; protein complex assembly; stress response |
| 2.9 | 2.9 | 7.1 | gi 81884348 | cytosol aminopeptidase (Leucine aminopeptidase) | 1.35 | 0.4766 | 8.74 | 1.18 | 0.15 | 1.68 | 1.42 | 0.0348 | 1.28 | proteolysis |
| 12.5 | 12.5 | 26.3 | gi 50402096 | elongation factor 1-alpha 2 (Statin S1) | 1.36 | 0.0064 | 1.22 | 0.99 | 0.51 | 1.23 | 1.36 | 0.0212 | 1.29 | translational regulation |
| 31.9 | 31.9 | 79.5 | gi 8810245 | voltage-dependent anion channel 1 (VDAC 1) | 1.41 | <0.0001 | 1.13 | 1.21 | 0.02 | 1.16 | 1.29 | 0.0005 | 1.14 | transport: anion transport |
| 7.9 | 8.0 | 8.1 | gi 6981424 | prosaposin | 1.61 | 0.0891 | 1.80 | 0.94 | 0.63 | 1.39 | 1.55 | 0.0357 | 1.48 | transport: lipid and fatty acid transport |
| 54.7 | 54.7 | 74.2 | gi 38014819 | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4 (Slc25a4) | 1.08 | 0.0210 | 1.07 | 1.15 | 0.05 | 1.11 | 1.14 | 0.0024 | 1.09 | transport: nucleoside, nucleotide and nucleic acid transport |
| 18.7 | 18.7 | 39.0 | gi 9798638 | glyceraldehyde-3-phosphate dehydrogenase (Gapdh) | 1,33 | 0.0786 | 1.26 | 1.11 | 0.91 | 1.20 | 1.40 | 0.0179 | 1.30 | unclassified |
| 13.7 | 13.7 | 67.1 | gi 68341999 | low molecular mass ubiquinone-binding protein | 1.21 | 0.0514 | 1.16 | 1.08 | 0.26 | 1.12 | 1.12 | 0.0344 | 1.11 | unclassified |
| 24.7 | 24.7 | 65.0 | gi 83302472 | ES1 protein homolog, mitochondrial precursor | 1.28 | 0.0260 | 1.16 | 1.08 | 0.29 | 1.15 | 1.25 | 0.0127 | 1.18 | no PANTHER hit |
| 14.9 | 14.9 | 61.3 | gi 535069 | muscle LIM protein | 1.59 | 0.0500 | 1.95 | 1.06 | 0.64 | 1.38 | 1.60 | 0.0500 | 1.47 | muscle development |
| 38.6 | 38.6 | 31.6 | gi 57824043 | myosin binding protein C, cardiac | 1.46 | 0.0191 | 1.16 | 1.17 | 0.01 | 1.14 | 1.33 | 0.0368 | 1.12 | muscle development/contraction |
| 30.5 | 30.5 | 30.5 | gi 34933197 | PREDICTED: similar to Amine oxidase [flavin-containing] A (Monoamine oxidase type A) (MAO-A) | 1.91 | 0.0002 | 1.20 | 0.91 | 0.37 | 1.20 | 1.85 | 0.0079 | 1.21 | oxidant stress |
| 17.4 | 17.5 | 34.7 | gi 109484674 | PREDICTED: similar to dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex) | 1.21 | 0.0266 | 1.12 | 0.99 | 0.76 | 1.14 | 1.29 | 0.0097 | 1.12 | metabolism: CHO metabolism |
| 18.9 | 18.9 | 46.5 | gi 56090293 | pyruvate dehydrogenase (lipoamide) beta | 0.97 | 0.7241 | 1.17 | 1.01 | 0.64 | 1.18 | 1.21 | 0.0096 | 1.15 | metabolism: CHO metabolism |
| 29.8 | 29.8 | 59.5 | gi 71051030 | pyruvate dehydrogenase E1 alpha 1 | 1.03 | 0.6054 | 1.11 | 0.99 | 0.69 | 1.09 | 1.18 | 0.0005 | 1.09 | metabolism: CHO metabolism |
| 2.9 | 2.9 | 25.4 | gi 149058974 | rCG44669 (cytochrome c oxidase, subunit VIIc; Cox7c) | 1.19 | 0.2121 | 1.35 | 1.42 | 0.05 | 1.30 | 1.26 | 0.0480 | 1.26 | unclassified |
| 29.6 | 29.7 | 56.0 | gi 149016520 | rCG50966 (3-oxoacid-CoA transferase 1 (OXCT1/SCOT) | 1.12 | 0.3615 | 1.27 | 1.08 | 0.46 | 1.23 | 1.33 | <0.0001 | 1.12 | metabolism: ketone metabolism |
| 60.9 | 60.9 | 67.6 | gi 116242506 | stress-70 protein, mitochondrial precursor (75 kDa glucose-regulated protein) (Heat shock 70 kDa protein 9) | 1.07 | 0.1432 | 1.12 | 1.02 | 0.39 | 1.10 | 1.13 | 0.0300 | 1.09 | protein folding; protein complex ass embly |
| 14.9 | 14.9 | 38.5 | gi 157820845 | Tu translation elongation factor, mitochondrial | 1.11 | 0.1522 | 1.16 | 0.96 | 0.40 | 1.20 | 1.29 | 0.0010 | 1.14 | no PANTHER hit |
| 32.4 | 32.4 | 55.1 | gi 55741544 | ubiquinol cytochrome c reductase core protein 2 | 1.07 | 0.2253 | 1.11 | 1.07 | 0.27 | 1.12 | 1.14 | 0.0108 | 1.10 | no PANTHER hit |
Proteins of interest were identified from 6 iTRAQ 8plex analyses and are grouped according to associated biological processes. Boldface indicates that the difference in expression was significant relative to adult ovary-intact control.