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Table 4.

Cardiac mitochondrial proteins with decreased abundance in aging and E2 deficiency

Unused Score Total Score % Coverage Accession # Protein Identified Quantitation Ratio Aged/Adult P Value Error Factor Quantitation Ratio Adult OVX/Adult P Value Error Factor Quantitation Ratio Aged OVX/Adult P Value Error Factor PANTHER Biological Process
17.9 17.9 42.9 gi 6978607 catalase 0.83 0.0458 1.20 0.82 0.02 1.11 0.84 0.0748 1.17 antioxidation and free radical removal
40.0 40.0 66.9 gi 56541110 acyl-Coenzyme A dehydrogenase, very long chain 1.02 0.8014 1.17 1.03 0.64 1.14 0.89 0.0237 1.11 metabolism: Acyl-CoA metabolism; electron transport
69.1 69.1 63.3 gi 60688124 hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit 0.83 0.0003 1.10 1.06 0.24 1.10 0.91 0.0235 1.09 metaboli sm: carbohydrate metabolism; beta-oxidation
38.3 38.3 66.3 gi 52782765 succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial precursor (Fp) 0.96 0.3486 1.10 1.02 0.67 1.10 0.91 0.0153 1.08 metabolism: carbohydrate metabolism; oxidative phosphoryl ation
10.9 10.9 44.6 gi 457929 ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (Atp5d) 0.85 0.0151 1.13 1.04 0.45 1.10 0.80 0.0067 1.16 metabolism: electron transport; cation trans port
52.2 52.2 54.6 gi 25742739 acyl-CoA synthetase long-chain family member 1 0.96 0.4715 1.13 1.00 0.89 1.11 0.89 0.0437 1.10 metabolism: fatty acid metabolism
6.0 6.0 20.4 gi 157818027 acyl-CoA synthetase short-chain family member 1 0.69 0.0002 1.15 0.82 0.51 1.76 0.81 0.0004 1.09 metabolism: fatty acid metabolism
17.5 17.5 25.2 gi 77993368 acyl-CoA synthetase family member 2 0.76 0.0200 1.23 0.94 0.60 1.31 1.01 0.8000 1.29 metabolism: fatty acid metabolism
50.7 50.7 77.8 gi 149027156 acetyl-CoA acyltransferase 2 1.05 0.3595 1.23 0.98 0.7540 1.15 1.25 0.02 1.15 metabolism: protein metabolism
20.1 20.1 44.3 gi 68837285 d-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor (3-hydroxybutyrate dehydrogenase) 0.80 0.0217 1.20 0.95 0.53 1.21 0.76 0.0402 1.30 metabolism: other metaboli sm
5.7 5.7 41.4 gi 157824071 NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 1.03 0.5880 1.13 0.92 0.60 1.36 0.83 0.0308 1.18 metabolism: oxidative phosphorylation
44.0 44.0 73.7 gi 55741424 NADH dehydrogenase (ubiquinone) flavoprotein 1, 51 kDa 0.97 0.3527 1.07 1.02 0.55 1.08 0.89 0.0095 1.09 metabolism: oxidative phosphorylation
5.4 5.4 35.8 gi 54145376 ATP synthase F0 complex subunit 8 0.83 0.0201 1.16 0.97 0.58 1.22 0.80 0.0426 1.24 metabolism: oxidative phosphorylation; cation transport
36.0 36.0 62.5 gi 81884378 cytochrome b-c1 complex subunit 1, mitochondrial precursor (Complex III subunit 1) 0.93 0.3321 1.14 0.98 0.61 1.12 0.89 0.0356 1.11 metabolism: proteolysis; electron transport
75.7 75.7 85.3 gi 71681130 subunit 0.94 0.0781 1.08 1.05 0.45 1.09 0.90 0.0258 1.09 metabolism: purine metabolism; electron transport
4.1 4.1 11.8 gi 53850596 succinate dehydrogenase complex, subunit C 0.88 0.3565 2.88 1.02 0.67 1.10 0.75 0.0005 1.00 metabolism: tricarboxylic acid pathway; electron transport
24.1 24.4 30.7 gi 6978543 ATPase, Na+/K+ transporting, alpha 1 polypeptide 0.84 0.0574 1.15 0.97 0.65 1.15 0.78 0.0038 1.16 transport: cation transport
10.6 10.6 38.8 gi 50927657 ATPase, Na+/K+ transporting, beta 1 polypeptide 0.78 0.0441 1.24 0.96 0.35 1.27 0.72 0.0135 1.19 transport: cation transport
50.0 50.0 37.3 gi 8392935 ATPase, Ca2+ transporting, myocardial, slow twitch 2 isoform a 0.81 0.0131 1.07 0.92 0.19 1.10 0.74 <0.0001 1.08 transport: cation transport; calcium ion homeostasis
25.1 25.1 40.1 gi 47718004 solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 3 (Slc25a3) 0.93 0.0730 1.08 0.94 0.27 1.18 0.89 0.0091 1.09 metabolism: electron tranport
32.2 32.2 89.6 gi 78099013 myoglobin 0.95 0.2943 1.10 1.15 0.26 1.14 0.90 0.0267 1.10 transport; blood circulation and gas exchange
7.6 7.6 84.3 gi 34786049 subunit 0.96 0.3948 1.11 1.07 0.45 1.10 0.81 0.0087 1.16 metabolism: electron tranport
16.5 16.5 71.1 gi 34849861 cytochrome c oxidase, subunit Vlc (Cox6c) 0.98 0.4443 1.06 0.89 0.01 1.13 0.90 0.0090 1.08 metabolism: electron tranport
2.2 3.2 18.3 gi 871525 calcium binding protein 0.77 0.4549 18.80 0.75 0.29 6.58 0.73 0.0354 1.25 no PANTHER hit
13.8 13.8 54.6 gi 599963 mitochondrial oxidative phosphorylation coupling factor 6 0.86 0.0088 1.12 1.02 0.63 1.11 0.81 0.0025 1.14 no PANTHER hit
16.3 16.3 34.6 gi 149042663 sarcalumenin (predicted), isoform CRA_a 0.80 0.0277 1.22 0.97 0.68 1.25 0.81 0.0240 1.19 no PANTHER hit

Proteins of interest were identified from 6 iTRAQ 8plex analyses and are grouped according to associated biological processes. Boldface indicates that the difference in expression was significant relative to adult ovary-intact control.