Table 4.
Cardiac mitochondrial proteins with decreased abundance in aging and E2 deficiency
| Unused Score | Total Score | % Coverage | Accession # | Protein Identified | Quantitation Ratio Aged/Adult | P Value | Error Factor | Quantitation Ratio Adult OVX/Adult | P Value | Error Factor | Quantitation Ratio Aged OVX/Adult | P Value | Error Factor | PANTHER Biological Process |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 17.9 | 17.9 | 42.9 | gi 6978607 | catalase | 0.83 | 0.0458 | 1.20 | 0.82 | 0.02 | 1.11 | 0.84 | 0.0748 | 1.17 | antioxidation and free radical removal |
| 40.0 | 40.0 | 66.9 | gi 56541110 | acyl-Coenzyme A dehydrogenase, very long chain | 1.02 | 0.8014 | 1.17 | 1.03 | 0.64 | 1.14 | 0.89 | 0.0237 | 1.11 | metabolism: Acyl-CoA metabolism; electron transport |
| 69.1 | 69.1 | 63.3 | gi 60688124 | hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), alpha subunit | 0.83 | 0.0003 | 1.10 | 1.06 | 0.24 | 1.10 | 0.91 | 0.0235 | 1.09 | metaboli sm: carbohydrate metabolism; beta-oxidation |
| 38.3 | 38.3 | 66.3 | gi 52782765 | succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial precursor (Fp) | 0.96 | 0.3486 | 1.10 | 1.02 | 0.67 | 1.10 | 0.91 | 0.0153 | 1.08 | metabolism: carbohydrate metabolism; oxidative phosphoryl ation |
| 10.9 | 10.9 | 44.6 | gi 457929 | ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (Atp5d) | 0.85 | 0.0151 | 1.13 | 1.04 | 0.45 | 1.10 | 0.80 | 0.0067 | 1.16 | metabolism: electron transport; cation trans port |
| 52.2 | 52.2 | 54.6 | gi 25742739 | acyl-CoA synthetase long-chain family member 1 | 0.96 | 0.4715 | 1.13 | 1.00 | 0.89 | 1.11 | 0.89 | 0.0437 | 1.10 | metabolism: fatty acid metabolism |
| 6.0 | 6.0 | 20.4 | gi 157818027 | acyl-CoA synthetase short-chain family member 1 | 0.69 | 0.0002 | 1.15 | 0.82 | 0.51 | 1.76 | 0.81 | 0.0004 | 1.09 | metabolism: fatty acid metabolism |
| 17.5 | 17.5 | 25.2 | gi 77993368 | acyl-CoA synthetase family member 2 | 0.76 | 0.0200 | 1.23 | 0.94 | 0.60 | 1.31 | 1.01 | 0.8000 | 1.29 | metabolism: fatty acid metabolism |
| 50.7 | 50.7 | 77.8 | gi 149027156 | acetyl-CoA acyltransferase 2 | 1.05 | 0.3595 | 1.23 | 0.98 | 0.7540 | 1.15 | 1.25 | 0.02 | 1.15 | metabolism: protein metabolism |
| 20.1 | 20.1 | 44.3 | gi 68837285 | d-beta-hydroxybutyrate dehydrogenase, mitochondrial precursor (3-hydroxybutyrate dehydrogenase) | 0.80 | 0.0217 | 1.20 | 0.95 | 0.53 | 1.21 | 0.76 | 0.0402 | 1.30 | metabolism: other metaboli sm |
| 5.7 | 5.7 | 41.4 | gi 157824071 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex 3 | 1.03 | 0.5880 | 1.13 | 0.92 | 0.60 | 1.36 | 0.83 | 0.0308 | 1.18 | metabolism: oxidative phosphorylation |
| 44.0 | 44.0 | 73.7 | gi 55741424 | NADH dehydrogenase (ubiquinone) flavoprotein 1, 51 kDa | 0.97 | 0.3527 | 1.07 | 1.02 | 0.55 | 1.08 | 0.89 | 0.0095 | 1.09 | metabolism: oxidative phosphorylation |
| 5.4 | 5.4 | 35.8 | gi 54145376 | ATP synthase F0 complex subunit 8 | 0.83 | 0.0201 | 1.16 | 0.97 | 0.58 | 1.22 | 0.80 | 0.0426 | 1.24 | metabolism: oxidative phosphorylation; cation transport |
| 36.0 | 36.0 | 62.5 | gi 81884378 | cytochrome b-c1 complex subunit 1, mitochondrial precursor (Complex III subunit 1) | 0.93 | 0.3321 | 1.14 | 0.98 | 0.61 | 1.12 | 0.89 | 0.0356 | 1.11 | metabolism: proteolysis; electron transport |
| 75.7 | 75.7 | 85.3 | gi 71681130 | subunit | 0.94 | 0.0781 | 1.08 | 1.05 | 0.45 | 1.09 | 0.90 | 0.0258 | 1.09 | metabolism: purine metabolism; electron transport |
| 4.1 | 4.1 | 11.8 | gi 53850596 | succinate dehydrogenase complex, subunit C | 0.88 | 0.3565 | 2.88 | 1.02 | 0.67 | 1.10 | 0.75 | 0.0005 | 1.00 | metabolism: tricarboxylic acid pathway; electron transport |
| 24.1 | 24.4 | 30.7 | gi 6978543 | ATPase, Na+/K+ transporting, alpha 1 polypeptide | 0.84 | 0.0574 | 1.15 | 0.97 | 0.65 | 1.15 | 0.78 | 0.0038 | 1.16 | transport: cation transport |
| 10.6 | 10.6 | 38.8 | gi 50927657 | ATPase, Na+/K+ transporting, beta 1 polypeptide | 0.78 | 0.0441 | 1.24 | 0.96 | 0.35 | 1.27 | 0.72 | 0.0135 | 1.19 | transport: cation transport |
| 50.0 | 50.0 | 37.3 | gi 8392935 | ATPase, Ca2+ transporting, myocardial, slow twitch 2 isoform a | 0.81 | 0.0131 | 1.07 | 0.92 | 0.19 | 1.10 | 0.74 | <0.0001 | 1.08 | transport: cation transport; calcium ion homeostasis |
| 25.1 | 25.1 | 40.1 | gi 47718004 | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 3 (Slc25a3) | 0.93 | 0.0730 | 1.08 | 0.94 | 0.27 | 1.18 | 0.89 | 0.0091 | 1.09 | metabolism: electron tranport |
| 32.2 | 32.2 | 89.6 | gi 78099013 | myoglobin | 0.95 | 0.2943 | 1.10 | 1.15 | 0.26 | 1.14 | 0.90 | 0.0267 | 1.10 | transport; blood circulation and gas exchange |
| 7.6 | 7.6 | 84.3 | gi 34786049 | subunit | 0.96 | 0.3948 | 1.11 | 1.07 | 0.45 | 1.10 | 0.81 | 0.0087 | 1.16 | metabolism: electron tranport |
| 16.5 | 16.5 | 71.1 | gi 34849861 | cytochrome c oxidase, subunit Vlc (Cox6c) | 0.98 | 0.4443 | 1.06 | 0.89 | 0.01 | 1.13 | 0.90 | 0.0090 | 1.08 | metabolism: electron tranport |
| 2.2 | 3.2 | 18.3 | gi 871525 | calcium binding protein | 0.77 | 0.4549 | 18.80 | 0.75 | 0.29 | 6.58 | 0.73 | 0.0354 | 1.25 | no PANTHER hit |
| 13.8 | 13.8 | 54.6 | gi 599963 | mitochondrial oxidative phosphorylation coupling factor 6 | 0.86 | 0.0088 | 1.12 | 1.02 | 0.63 | 1.11 | 0.81 | 0.0025 | 1.14 | no PANTHER hit |
| 16.3 | 16.3 | 34.6 | gi 149042663 | sarcalumenin (predicted), isoform CRA_a | 0.80 | 0.0277 | 1.22 | 0.97 | 0.68 | 1.25 | 0.81 | 0.0240 | 1.19 | no PANTHER hit |
Proteins of interest were identified from 6 iTRAQ 8plex analyses and are grouped according to associated biological processes. Boldface indicates that the difference in expression was significant relative to adult ovary-intact control.