Fig. 6.

Glycoprotein nonmestastatic melanoma protein b (GPNMB) expression in the lungs. A: Western analysis of GPNMB protein levels in the CON and hyperoxia-exposed lungs. B: immunostaining in the lungs. For colocalization of GPNMB with Clara cell secretory protein (CCSP) (arrows), 2 consecutive lung sections were probed with anti-CCSP and anti-GPNMB antibodies. For colocalization of GPNMB and surfactant protein (SP)-C, double-labeling was performed with anti-SP-C (red) and anti-GPNMB (green) antibodies. GPNMB was also detected in macrophages (*). Arrowheads: overlap of GPNMB and SP-C. Square areas are enlarged in the panels immediately to the right. All scale bars: 40 μm.