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Effect of miR-150 on GPNMB expression. 293FT and H441 were transduced with an adenovirus expressing miR-150 (Ad-miR-150) or adenovirus control (Ad-Con) at multiplicities of infectivity (MOI) of 5, 20, and 100. A, C: mRNA levels of GPNMB as determined by qRT-PCR, n = 3 independent preparations, each assay performed in duplicate. *P < 0.05 vs. Ad-Con at the same MOI. B, D: Western blot analysis of GPNMB protein levels.
