Fig. 7.

Skeletal muscle neuronal NO synthase (nNOS) protein content determined by Western blot analyses from the vastus lateralis muscle of nonobese (n = 13) and obese (n = 14) sedentary subjects. Representative nNOS Western blot (A), and comparison between nonobese and obese skeletal muscle nNOS protein content (B). The representative nNOS Western blot has an accompanying representative Ponceau S stained blot, which is shown in Fig. 5B. The representative eNOS and nNOS blots share the same Ponceau S blot because commercial stripper was utilized so that membranes could be reprobed. No statistically significant difference was observed.