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. 2012 Jun 28;113(8):1311–1322. doi: 10.1152/japplphysiol.00617.2012

Fig. 6.

Fig. 6.

Colocalization of ASIC3 and peripherin. Fluorescence immunohistochemistry was employed to examine double labeling of ASIC3 and peripherin. In this experiment, peripherin was used to label DRG neurons that project C-fiber. A: representative photomicrographs show ASIC3 and peripherin staining in DRG neurons of a control limb (top panel) and a limb with NGF infusion (bottom panel). Arrows indicate representative cells positive for both ASIC3 and peripherin after they were merged. The number of double labeling DRG neurons is greater in infused limbs than that in control limbs. Scale bar = 50 μm. B: average data for colocalization of ASIC3 and peripherin, showing that the percentage of double labeling DRG neurons for ASIC3 and peripherin is greater in NGF infusion limbs than that in control limbs. *P < 0.05 vs. control (one-way ANOVA). No differences were observed in the number of peripherin positive DRG neurons in control and NGF infusion groups.