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. 2012 Jul 10;23(10):1090–1100. doi: 10.1089/hum.2012.080

FIG. 1.

FIG. 1.

The effect of cytokine stimulation on lentiviral transduction efficiency. Freshly isolated primary natural killer (NK) cells were cultured in CellGro SCGM medium with 10% human serum in the presence of cytokines as indicated in the figure. After 24 hr of stimulation, NK cells were transduced at an multiplicity of infection (MOI) of 20 using the VSV-G pseudotyped lentiviral “gene ontology” (LeGO)-G2 vector. Seventy-two hours post-transduction, acquisition of enhanced green fluorescent protein (eGFP) expression was carried out by flow cytometry. Data from five independent donors. *p<0.05; **p<0.01; one-way ANOVA repeated measures.