FIG. 4.

Recognition of GSCs by anti-EGFRvIII CAR-transduced T cells. The third-generation anti-EGFRvIII CAR vector (139-28BBZ) was used to transduce T cells from two donors (donors 4 and 5) along with a GFP-expressing vector (GFP) and a CAR vector targeting ERBB2. Transduced T cells were cocultured with wild-type EGFR-engineered U251 cells (U251-EGFRwt) or EGFR variant III engineered U251 cells (U251-EGFRvIII) and GSC lines 1228, 308, and 822. IFN-γ values were determined by ELISA (shown in pg/mL, mean of triplicate determinations). Data are representative of three independent experiments. Differences between recognition of EGFRvIII-transduced U251 versus EGFRwt-U251 were statistically significant (p<0.01, Student's t test).