FIG. 3.

Three-reporter gene monitoring in multiple tissues after Cre vector injection. (A) Imaging for luciferase activity in male hybrid mice injected by the intravenous or intranasal route with the indicated amounts of scAAV9-Cre 20 days postinjection. (B) Quantification of luciferase activity in the same mice by measuring total photon flux over 10 min per mouse. (C) Confocal images of select tissue sections from mice injected with the indicated amounts of scAAV9-Cre by the indicated routes. Tissues are counterstained for nuclei with DAPI. Nontransgenic mice do not express fluorescent proteins and show only DAPI stain. Positive control mice encode only mG, which is constitutively expressed. Original magnifications are 400x.