FIG. 4.

Transduction profile of scAAV9-Cre in the glomeruli of female hybrid mice by immunofluorescence staining 21 days after intravenous injection with 1×10¹² GC. (A) Diagram depicting structural relationship of blood flow, podocytes, endothelial cells, and proximal tubules in kidney. (B) Confocal image of PFA-fixed kidney cryosections (thickness, 7 μm) at magnifications of ×100 and ×400 with DAPI-stained nuclei (405 nm emission, blue), transduced cells (488 nm emission, green), and nontransduced cells (563 nm emission, red). (C) Immunofluorescence confocal imaging and overlays of PFA-fixed kidney cryosections (thickness, 18 μm) for denoted cell types (647 nm emission, purple); DAPI counterstain not shown. EpCAM, epithelial cell adhesion molecule; Syn, synaptopodin.