FIG. 5.
Transduction profile of scAAV8-Cre in female hybrid mice 21 days after intravenous injection with 1×1012 GC. PFA-fixed kidney cryosections. (A) Luciferase image and confocal images ( ×400) of cryosections taken from various tissues (thickness, 7 μm) at ×100 and ×400 magnification with DAPI-stained nuclei (405 nm emission, blue), transduced cells (488 nm emission, green), and nontransduced cells (563 nm emission, red). (B) Immunofluorescence imaging by confocal of kidney tissue sections (thickness, 18 μm) for denoted cell types (647 nm emission, purple); DAPI counterstain not shown. EpCAM, epithelial cell adhesion molecule; Syn, synaptopodin.