Figure 2.

CD206⁺CD1c⁺ DDCs can be distinguished from CD206⁺CD209⁺ skin macrophages in embryonic skin. (a) Arrows indicate CD206⁺CD209⁺ cells, and arrowheads indicate CD1c⁺ DCs in all indicated age groups. One of at least four experiments per group is shown. Bars=50 μm. (b) Dot graph shows the increase of CD45⁺CD1c⁺ cells in developing skin using flow cytometry. (c) Shown are relative numbers of CD1c⁺ and CD209⁺ dermal cells at selected developmental time points analyzed by immunoflourescence (n=4–5 per group). DC, dendritic cell; DDC, dermal dendritic cell; EGA, estimated gestational age.