Figure 3.

CD11c⁺ leukocytes are present throughout skin development. (a, b) Multiparameter flow cytometry of freshly isolated single cell suspensions of embryonic, fetal, and adult skin was performed by incubation with mAbs against the cell surface markers indicated. Gates in dot plots were set according to isotype-matched control staining. Dead cells were excluded by 7-amino-actinomycin-D uptake. Dot plots are representative of 3–6 experiments. (b) CD45⁺HLA-DR^high (R1), CD45⁺HLA-DR^weak (R2), and CD45⁺HLA-DR⁻ (R3) cells from the indicated age groups are compared with regard to expression of CD11c and CD1c (lower panels). EGA, estimated gestational age.