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. 2012 Nov 1;139(21):3938–3949. doi: 10.1242/dev.081133

Fig. 4.

Fig. 4.

Sox1 lineage tracing marks a subset of putative stem cells, as well as newly born neuroblasts, granule neurons and astrocytes. Sox1-tTA; LC-1; R26eYFP mice were sacrificed at 3.5 months of age and brains were harvested and sectioned into 50 μm slices for antibody staining and imaging by confocal microscopy. (A) GFAP (red), GFP (green) and Sox1 (blue) staining of the dentate gyrus. (B) Sox2 (red), GFP (green) and DNA (ToPro3, blue) staining of the dentate gyrus. Note that grainy GFP staining above Sox2-positive cells is background. (C) GFAP (red), GFP (green) and BrdU (blue) staining of the dentate gyrus. (D) GFAP (red), GFP (green) and DNA (ToPro3, blue) staining of an astrocyte within the hilus. Arrowheads mark cells that are positive for all three antibodies. All mice were taken off of doxycycline at 1.5 months. Scale bars: 10 μm.