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. 2012 Nov 1;139(21):4029–4039. doi: 10.1242/dev.082776

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Fig. 3. — Shark and Rac1 are required in follicle cells for proper engulfment. Egg chambers from starved flies express G71 GFP in the germline, stained with α-Dlg (magenta) and DAPI (cyan). (A-A″) GRI-GAL4 alone shows normal progression of mid-oogenesis PCD. (B-B″) UAS-shark^dsRNA/+; GRI-GAL4/+ shows defective clearance of nurse cells (arrow). Follicle cell (FC) membranes do not enlarge and GFP is largely absent in the FCs (arrowhead in B′). FCs are pyknotic (arrowheads in B″), as in drpr^Δ5 mutants. (C-C″) UAS-Rac1^DN/+; GRI-GAL4/+ shows same phenotype. Scale bar: 50 μm. (D) Quantification of the unengulfed area. Data are mean±s.e.m. *P<0.05, ***P<0.001.