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. 2012 Nov 1;139(21):3973–3985. doi: 10.1242/dev.081596

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© 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 2. — Targeting of Erg^Δ^Ex3/+ and Erg^Δ^Ex4/+ loci. (A) The wild-type murine Erg locus, the targeting construct, and targeted locus with exon 3 replaced by the lacZ-Neo reporter cassette. The numbered boxes represent Erg exons. Bold lines indicate the arms of homology. The positions of Southern blotting probes are indicated. (B) Southern analysis to identify Erg^ΔEx3/+ ES cell clones using 5′ and 3′ probes that distinguish NheI fragments in the wild-type and targeted alleles. (C) Erg wild-type locus, the targeting construct, and targeted locus with exon 4 replaced by the lacZ-Neo reporter cassette. (D) Southern analysis to identify Erg^ΔEx4/+ ES cell clones using 5′ and 3′ probes that distinguish BssSI and NheI fragments in the wild-type and targeted allele, respectively.