Figure 3.

pERK2 and p-p38 levels are increased in the striatum of R6/1 mice. pERK2 (A) and p-p38 (B) levels were analyzed by Western blot of protein extracts obtained from the striatum of 8- to 30-week-old WT and R6/1 mice. Representative immunoblots show protein levels of pERK2, ERK2, p-p38, p38, and α-tubulin in WT and R6/1 mice at 8, 20, and 30 weeks of age. The graphs show that the striatal levels of pERK2 and p-p38 are significantly increased in R6/1 mice with respect to their littermate controls at 20 and 30 weeks of age. Values (obtained by densitometric analysis of Western blot data) are expressed as percentage of age-matched WT mice (pERK2/ERK2 and p-p38/p38 ratio after normalization with α-tubulin) and shown as mean ± SEM (n = 6). Data were analyzed by Student's t test. *p < 0.05, **p < 0.01, and ***p < 0.001 compared with WT mice.