Figure 9.

Changes in STEP expression but not in STEP phosphorylation are reverted by suppressing the mhtt transgene expression in Tet/HD94 mice striatum. pSTEP₆₁, STEP₆₁, and STEP₄₆ protein levels were analyzed by Western blot in the striatum of 22-month-old WT and Tet/HD94 mice, either with no pharmacological intervention (Gene-ON) or after 5 months of mhtt transgene shutdown by doxycycline administration (Gene-OFF). A, B, The graphs show the densitometric measures of STEP₆₁ and STEP₄₆ normalized to α-tubulin (A) and the pSTEP₆₁/STEP ratio after normalization with α-tubulin (B). Results are expressed as percentages of WT ± SEM. Data were analyzed by one-way ANOVA, followed by Bonferroni's post hoc test. *p < 0.05 compared with WT mice; ^#p < 0.05 compared with Tet/HD94 gene-ON mice.