FIG. 6.

(A) SSEA1-positive cells (green) in transient ES cell cultures. All the cells are negative for OCT4 (red) and SOX2 (light blue). Cell nuclei are shown in blue (DAPI). (B) SOX2-positive cell colonies (DAPI, blue; SOX2, green; merged image). (C) Nestin-positive colonies (DAPI, blue; nestin, light blue). (D) Neuronal precursors. Nuclei are shown in blue (DAPI) and neurites in red (merged image). (E) Diagram showing markers that define the transition between blastomeres, ES cells, and neuronal precursors. Color images available online at www.liebertonline.com/zeb