Figure 1.

Characterization of HCT116 and its tri- and tetrasomic derivatives. (A) Chromosome paints of used tri- and tetrasomic cell lines. Bar—10 μm. (B) Growth curves of tri- and tetrasomic cell lines in comparison with their diploid counterparts. Each point represents the mean with standard deviation of three independent experiments. (C) Cell-cycle progression of HCT116 (left panel) and HCT116 5/4 (right panel) after release from thymidine block, analyzed by flow cytometry. The major delay occurs in the G1 and the S phase. The length of each cell-cycle phase is indicated above the graph. Population was considered to enter a specific phase of cell cycle if at least 50% of cells showed corresponding DNA content. See also Supplementary Figure S1. Source data is available for this figure in the Supplementary Information.

Source Data for Figure 1B ^{(22.5KB, xls)}

Source data for Figure 1C ^{(23.5KB, xls)}