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. 2012 Aug 30;13(9):10863–10879. doi: 10.3390/ijms130910863

Figure 4.

Figure 4

The expression of human CD4 mRNA in IDO expressing C8166 cells. C8166 cells were infected with either pAd-EGFP or pAd-IDOEGFP, and at 60 h post transfection, the total RNA was extracted and the cDNA was synthesized as described in the Materials and Methods section. Panel A illustrate the total RNA from untreated (control), pAd-EGFP or pAd-IDOEGFP infected C8166 cells, extracted as described in methods and subjected to electrophoresis through a native 1.5% agarose gel; Panel B displays melting curves of RT-PCR product generated using GeneAmp Ster One Plus SDS software.