Figure 2.

ER prevents 6-OHDA-induced apoptosis in SH-SY5Y cells. SH-SY5Y cells were incubated with various concentrations of ER for 24 h and then treated with 6-OHDA (200 μmol/L) for 2 h at 37 °C. After a further 15 h of incubation in medium without 6-OHDA, the apoptotic cells were determined by Annexin V staining and fluorescence microscopy as described in the Experimental Section. The values are expressed as a percentage of total number of apoptotic cells and shown as mean ± SEM of three/four independent experiments. ** p < 0.01, *** p < 0.001 versus untreated cells at ANOVA with Dunnett post hoc test.