Table 1.
Unigene No. | Length (bp) | Putative Function | Organism of Best Homology | Accession No. | E-Value |
---|---|---|---|---|---|
Metabolism (19) | |||||
UN018 | 706 | Abscisic stress ripening protein | V. pseudoreticulata | ABC86744 | 5 × 10−21 |
UN022 | 548 | GDP-d-glucose phosphorylase | A. thaliana | NP_200323 | 2 × 10−19 |
UN044 a | 562 | S-adenosylmethionine synthetase | R. communis | XP_002512570 | 4 × 10−101 |
UN102 | 369 | ATP-dependent peptidase/ATPase/metallopeptidase | A. thaliana | NP_564563 | 3 × 10−59 |
UN127 | 829 | Malate dehydrogenase | N. tabacum | CAC12826 | 3 × 10−139 |
UN145 | 540 | Putative cinnamoyl-CoA reductase | R. communis | XP_002533639 | 4 × 10−19 |
UN173 | 250 | ATP-dependent peptidase/ATPase/metallopeptidase | A. thaliana | NP_568604 | 9 × 10−6 |
UN178 | 592 | GDSL-motif lipase/hydrolase family protein | A. thaliana | NP_190416 | 7 × 10−59 |
UN183 | 452 | Malate dehydrogenase | V. vinifera | AAF69802 | 6 × 10−75 |
UN230 | 247 | Aldo/keto reductase, putative | R. communis | XP_002535506 | 8 × 10−18 |
UN240 | 416 | S-adenosylmethionine-dependent methyltransferase | A. thaliana | NP_173458 | 2 × 10−63 |
UN262 | 443 | HMGB3 (High mobility group B 3) | A. thaliana | NP_001031075 | 3 × 10−27 |
UN266 | 558 | NADH dehydrogenase subunit 2 | A. thaliana | NP_085584 | 5 × 10−16 |
UN035 | 395 | Naringenin-chalcone synthase | Humulus lupulus | CAK19317 | 2 × 10−45 |
UN040 | 645 | Cinnamate-4-hydroxylase | Canarium album | ACR10242 | 2 × 10−99 |
UN118 | 204 | Flavonol synthase | V. vinifera | BAE75808 | 1 × 10−26 |
UN166 | 333 | Phenylalanine ammonia-lyase | Camellia oleifera | ACT21093 | 8 × 10−50 |
UN182 a | 616 | Chalcone-flavanone isomerase | A. thaliana | NP_568154 | 8 × 10−41 |
UN260 a | 307 | Phenylalanin ammonia-lyase | V. vinifera | ABM67591 | 3 × 10−25 |
Photosynthesis and Energy (15) | |||||
UN001 | 618 | Ribulose-1,5-bisphosphate carboxylase/oxygenase activase 1 | Acer rubrum | ABI94077 | 2 × 10−108 |
UN017 | 562 | Ribulose bisphosphate carboxylase/oxygenase activase 1 | R. communis | XP_002532996 | 2 × 10−89 |
UN020 | 455 | Photosystem I reaction center subunit II, chloroplast precursor | R. communis | XP_002516772 | 4 × 10−56 |
UN023 | 624 | Photosystem II 22 kDa protein, chloroplast precursor | R. communis | XP_002513761 | 1 × 10−68 |
UN025 | 438 | Ribulose bisphosphate carboxylase/oxygenase activase 1 | R. communis | XP_002532996 | 3 × 10−73 |
UN052 | 365 | Chloroplast photosystem II 10 kDa polypeptide | Jatropha curcas | ADB93062 | 4 × 10−41 |
UN086 | 255 | Photosystem I reaction center subunit II, chloroplast precursor | R. communis | XP_002516772 | 3 × 10−17 |
UN093 | 298 | Putative fructose-1,6-bisphosphatase | R. communis | XP_002527886 | 3 × 10−28 |
UN095 | 348 | Ribulose-1,5-bisphophate carboxylase/oxygenase activase 1 | V. pseudoreticulata | ABC86738 | 5 × 10−47 |
UN098 | 571 | Putative fructose-bisphosphate aldolase | R. communis | XP_002512993 | 2 × 10−100 |
UN106 | 268 | Ribulose-1,5-bisphosphate carboxylase/oxygenase activase 1 | Gossypium hirsutum | ABB20913 | 2 × 10−20 |
UN121 | 371 | Chlorophyll A/B binding protein | R. communis | XP_002533251 | 1 × 10−45 |
UN198 | 312 | Light harvesting chlorophyll a/b-binding protein | Nicotiana sylvestris | BAA25394 | 2 × 10−35 |
UN241 | 189 | Light-harvesting complex II protein Lhcb1 | Populus trichocarpa | XP_002307725 | 4 × 10−11 |
UN255 | 324 | Chlorophyll A/B binding protein | R. communis | XP_002519950 | 5 × 10−44 |
Disease/Defence (22) | |||||
UN016 | 477 | Stress-induced cysteine proteinase | Lavatera thuringiaca | AAB62937 | 4 × 10−66 |
UN033 | 427 | Similar to pathogenesis-related protein 10.3 | V. vinifera | XP_002274617 | 1 × 10−51 |
UN040 | 645 | Cytochrome P450 | Populus trichocarpa | XP_002325637 | 3 × 10−98 |
UN073 a | 351 | Thaumatin-like protein | V. vinifera | AAQ10092 | 7 × 10−67 |
UN075 | 346 | Glutathione S-transferase 3 | Papaver somniferum | AAF22519 | 1 × 10−8 |
UN077 | 450 | Chaperone protein dnaJ | R. communis | XP_002514419 | 3 × 10−68 |
UN079 | 294 | Responsive to dehydration 19 | A. thaliana | NP_568052 | 2 × 10−43 |
UN096 | 755 | Lipid transfer protein isoform 4 | V. vinifera | AAO33394 | 3 × 10−47 |
UN170 | 468 | Lipid transfer protein family protein | Tamarix hispida | ACM78616 | 6 × 10−8 |
UN205 | 470 | Lipid transfer protein family protein | A. thaliana | NP_680546 | 5 × 10−31 |
UN226 | 537 | Lipid transfer protein family protein | A. thaliana | NP_565348 | 2 × 10−33 |
UN115 | 574 | Bacterial-induced peroxidase precursor | G. hirsutum | AAD43561 | 1 × 10−71 |
UN116 a | 769 | Similar to senescence-associated protein | A. thaliana | BAD42919 | 3 × 10−70 |
UN119 | 698 | Catalase 1 | N. tabacum | AAB71764 | 2 × 10−80 |
UN174 a | 443 | Class IV chitinase | N. tabacum | BAF44533 | 3 × 10−41 |
UN179 | 318 | Proline-rich protein | Solanum tuberosum | CAA04449 | 1 × 10−22 |
UN209 | 212 | Harpin-induced family protein | A. thaliana | NP_190008 | 1 × 10−22 |
UN219 | 341 | Responsive to dehydration 21 | A. thaliana | NP_564497 | 2 × 10−44 |
UN233 | 465 | Proline-rich protein | G. hirsutum | ABM05952 | 2 × 10−16 |
UN239 | 240 | Chitinase-like protein | G. hirsutum | AAQ56598 | 2 × 10−35 |
UN058 | 474 | 2OG-Fe(II) oxygenase family protein | A. thaliana | NP_192787 | 1 × 10−50 |
UN043 | 581 | Ascorbate peroxidase 3 | A. thaliana | NP_195226 | 4 × 10−67 |
Protein synthesis and fate (7) | |||||
UN004 | 372 | 40S ribosomal protein S17 | A. thaliana | NP_196100 | 1 × 10−41 |
UN010 | 252 | 26S proteasome regulatory subunit | A. thaliana | AAP86657 | 2 × 10−39 |
UN134 a | 468 | Coronatine insensitive 1 (COI1) | A. thaliana | ABR45948 | 2 × 10−47 |
UN135 a | 356 | F-box family protein | A. thaliana | NP_199429 | 2 × 10−31 |
UN203 a | 784 | Jasmonate ZIM-domain protein 2 | N. tabacum | BAG68656 | 5 × 10−43 |
UN214 | 352 | Putative 40S Ribosomal protein | O. sativa | AAK92638 | 3 × 10−39 |
UN254 | 229 | Aspartyl protease family protein | A. thaliana | NP_566966 | 3 × 10−15 |
Signal transduction (8) | |||||
UN005 | 653 | Putative zinc finger protein | O. sativa | BAD87736 | 1 × 10−24 |
UN008 | 415 | CIPK2; protein serine/threonine kinase | A. thaliana | NP_196324 | 2 × 10−11 |
UN031 | 410 | Serine/threonine phosphatase | A. thaliana | NP_188632 | 5 × 10−29 |
UN151 | 332 | CIPK21; protein serine/threonine kinase | A. thaliana | NP_568860 | 3 × 10−29 |
UN211 | 636 | Lipid phosphate phosphatase 3 | A. thaliana | NP_001078096 | 2 × 10−35 |
UN216 | 447 | GTP binding/phospholipase activator | A. thaliana | NP_191788 | 1 × 10−79 |
UN217 | 508 | Rab-type small GTP-binding protein | Cicer arietinum | BAA76423 | 2 × 10−49 |
UN238 | 543 | Phosphoribulose kinase, putative | R. communis | XP_002519002 | 3 × 10−82 |
Transport (12) | |||||
UN011 | 324 | NRAMP3; transmembrane transporter | A. thaliana | NP_179896 | 6 × 10−45 |
UN029 | 862 | Plasma membrane aquaporin | V. vinifera | AAF80557 | 2 × 10−148 |
UN030 | 910 | Antiporter/triose-phosphate transmembrane transporter | A. thaliana | NP_851138 | 1 × 10−144 |
UN042 | 584 | Putative sorbitol transporter | Prunus cerasus | AAM44082 | 7 × 10−91 |
UN067 | 647 | Non-specific lipid transfer protein | V. vinifera | ABA29446 | 2 × 10−36 |
UN088 | 249 | Plasma membrane intrinsic protein | A. thaliana | AAB65787 | 3 × 10−38 |
UN129 | 401 | ABC transporter family protein | Populus trichocarpa | XP_002331473 | 6 × 10−60 |
UN136 | 478 | ABC transporter-like | A. thaliana | BAF01914 | 3 × 10−83 |
UN231 | 565 | ABC transporter family protein | Populus trichocarpa | XP_002308160 | 7 × 10−56 |
UN164 | 509 | Polyol transporter | Populus trichocarpa | XP_002313809 | 4 × 10−68 |
UN223 | 269 | Nitrate transporter | Malus hupehensis | ACN72639 | 2 × 10−34 |
UN221 | 329 | Solute symporter family protein | A. thaliana | NP_199351 | 5 × 10−42 |
Transcription (4) | |||||
UN014 | 517 | Transcription activator/transcription factor | A. thaliana | NP_197904 | 2 × 10−18 |
UN064 | 446 | Transcript elongation factor | A. thaliana | NP_181390 | 5 × 10−27 |
UN065 | 443 | IAA16; transcription factor | A. thaliana | NP_187124 | 3 × 10−46 |
UN167 | 430 | DNA binding/transcription factor | A. thaliana | NP_172094 | 1 × 10−21 |
Clones selected for further qRT-PCR analysis are marked with an asterisk.