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. 2012 Sep 14;13(9):11530–11542. doi: 10.3390/ijms130911530

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Downmodulation of AR and AR-V in 22Rv1 cells is due to sorafenib induced proteasomal degradation. 22Rv1 cells were incubated with the proteasome inhibitor MG132 (5 μM) for 60 min followed by treatment with sorafenib (5 μM) for 18 h. Subsequently cell extracts were analyzed by Western blot analysis (AR: androgen receptor; AR-V, ARΔLBD generated by alternative splicing; β-actin: loading control; ctrl: untreated control; SORA: sorafenib; MG132: proteasome inhibitor). AR, AR-V and β-actin levels were quantified by densitometry and expressed as fold-change of AR/β-actin or AR-V/β-actin control (ctrl) levels which were set at 1.00.