Figure 2.

The figure provides evidence that the marked protective effect of 100 nM α-T is revealed when pre-incubation of PC12 cells with it is performed for 3–18 h, prior to the exposure to H₂O₂. In this figure, the results of one typical experiment (from four replicates) are presented as means ± SEM of 2–3 parallel determinations. PC12 cells were pre-incubated for 3 h (A); 6 h (B); 12 h (C) or 18 h (D) with α-T and then exposed to 0.2 mM H₂O₂ for 24 h. The differences are significant by one-way ANOVA followed by Tukey’s multiple comparison test: * as compared to control values, p < 0.01; ^x as compared to the effect of H₂O₂ alone, p < 0.05; ^# as compared to the effect of α-T at all higher concentrations, p < 0.05.