Figure 5.

The figure shows the effect of pre-incubation for 18 h with α-T, prior to PC12 cell exposure to 0.2 mM H₂O₂ for 24 h on the relative number of PC12 cells in late apoptosis (% of the total cell number). The results of one typical experiment from 5 replicates are presented. The relative value of subG1 hypodiploid peak (left on the histograms) corresponds to the relative number of PC12 cells in late apoptosis. In the figure: (A) control; (B) H₂O₂; (C) H₂O₂ + pre-incubation with 50 μM α-T; (D) H₂O₂ + pre-incubation with 100 nM α-T. In the control sample, the relative number of cells in late apoptosis (A) is lower than 1%, but exposure to H₂O₂ increases this peak to 32% (B). However, pre-incubation with 50 μM and 100 nM α-T decreases the relative value of the subG1 hypodiploid peak from 32% (B) to 3% and 4% of the total cell number, respectively (C,D).