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. 2012 Sep 14;13(9):11543–11568. doi: 10.3390/ijms130911543

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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The figure shows the effect of hydrogen peroxide and pre-incubation with α-T on the activity (pERK 1/2 level) and expression of ERK 1/2 in PC12 cells. PC12 cells were pre-incubated with 100 nM and 100 μM α-T (or without it) for 18 h and then exposed to 0.3 mM H₂O₂. The results of immunoblotting obtained in one typical experiment (from five experiments made) are shown in (A,B), (A) 100 nM α-T; (B) 100 μM α-T. The results of five experiments are shown in (C,D) as means ± SEM; (C) 100 nM α-T; (D) 100 μM α-T. Red lines with square data points show effect of H₂O₂ alone, black lines with diamond data points show the effect of H₂O₂ after pre-incubation with α-T. In (C,D): HP is an abbreviation for hydrogen peroxide. alpha-T is an abbreviation for α-tocopherol * the differences are significant according to Students’ paired t test, as compared to the level of pERK 1/2 in the PC12 cells exposed to α-T and H₂O₂, p < 0.05.

The figure shows the effect of hydrogen peroxide and pre-incubation with α-T on the activity (pERK 1/2 level) and expression of ERK 1/2 in PC12 cells. PC12 cells were pre-incubated with 100 nM and 100 μM α-T (or without it) for 18 h and then exposed to 0.3 mM H₂O₂. The results of immunoblotting obtained in one typical experiment (from five experiments made) are shown in (A,B), (A) 100 nM α-T; (B) 100 μM α-T. The results of five experiments are shown in (C,D) as means ± SEM; (C) 100 nM α-T; (D) 100 μM α-T. Red lines with square data points show effect of H₂O₂ alone, black lines with diamond data points show the effect of H₂O₂ after pre-incubation with α-T. In (C,D): HP is an abbreviation for hydrogen peroxide. alpha-T is an abbreviation for α-tocopherol * the differences are significant according to Students’ paired t test, as compared to the level of pERK 1/2 in the PC12 cells exposed to α-T and H₂O₂, p < 0.05.