Table 2.
List of 13 BAC clones used for mapping of the proximal boarder of FRA10F.
| BAC clone | Location on chromosome 10 (Mb) | Proximal to FRA10F | Supposed breakpoint | Inside FRA10F | Distal to FRA10F |
|---|---|---|---|---|---|
| RP11-198M6 | 121.577.828-121.765.053 | 1 | - | - | - |
| RP11-323P17 | 122.469.430-122.626.379 | 1 | - | - | - |
| RP11-105F10 | 123.764.471-123.939.147 | 1 | - | - | - |
| RP11-296H2 | 123.939.148-124.083.531 | 1 | - | - | - |
| RP11-436O19 | 124.267.798-124.268.644 | 1 | - | 1 | - |
| RP11-481L19 | 124.268.654-124.444.805 | 1 | - | - | - |
| RP11-564D11 | 124.592.597-124.787.891 | 1 | - | - | - |
| RP11-162A23 | 124.799.045-124.978.916 | 1 | - | - | - |
| RP11-391M7 | 125.381.493-125.576.300 | 1 | - | - | - |
| RP11-435D11 | 125.909.463-126.009.423 | - | 1 | - | - |
| RP13-238F13 | 126.009.424-126.190.394 | - | - | 1 | - |
| RP11-124H7 | 127.445.890-127.599.588 | - | 6 | - | - |
| RP11-179O22 | 128.076.478-128.246.854 | - | 1 | 3 | - |
Notes: BAC clones from 10q26.13–26.2 spanning 8 Mb were selected from the UCSC database (http://genome.ucsc.edu). The frequency of FRA10F in one individual was 4 in 500 metaphase spreads (0.8%) compared to 1 in 500 (0.2%) in the second individual. FATS-containing BAC clone RP11-179O22 was hybridized on four different metaphase spreads in FISH analysis, showing that FATS was located once at the breakpoint and three times within FRA10F. Therefore, the location of FRA10F is refined to 10q26.13–q26.2 (Figure 2).