A: Nestin immunostaining of Neurosphere Sections
Hypothalamic NPC from 1 day old Control () and LBW () newborn males were cultured in complete media. Neurospheres were sectioned (5 µm) and immunostained with nestin, Neu and GFAP. Images of LBW and Control are shown at 40× magnification.
B: Basal Hypothalamic NPC Proliferation
Hypothalamic NPC from 1 day old Control () and LBW () newborn males were cultured in complete media. Live images (magnification ×20), basal cell proliferation rate and nestin protein expression of LBW and Control NPCs. Values are fold change (mean ± SE); * P < 0.05 LBW vs. Control.
C: Basal Hypothalamic NPC Differentiation
Hypothalamic NPC from 1 Control () and LBW () newborn males were cultured in differentiating media. NPC were harvested and protein expression of neuronal marker (Tuj1)) and astrocyte marker (GFAP) were determined. Values are fold change (mean ± SE); * P < 0.05 LBW vs. Control.