Skip to main content
. Author manuscript; available in PMC: 2012 Oct 16.
Published in final edited form as: Brain Res. 2011 Jan 6;1378:29–42. doi: 10.1016/j.brainres.2010.12.080

Figure 2.

Figure 2

Figure 2

Figure 2

A: Nestin immunostaining of Neurosphere Sections

Hypothalamic NPC from 1 day old Control (Inline graphic) and LBW (Inline graphic) newborn males were cultured in complete media. Neurospheres were sectioned (5 µm) and immunostained with nestin, Neu and GFAP. Images of LBW and Control are shown at 40× magnification.

B: Basal Hypothalamic NPC Proliferation

Hypothalamic NPC from 1 day old Control (Inline graphic) and LBW (Inline graphic) newborn males were cultured in complete media. Live images (magnification ×20), basal cell proliferation rate and nestin protein expression of LBW and Control NPCs. Values are fold change (mean ± SE); * P < 0.05 LBW vs. Control.

C: Basal Hypothalamic NPC Differentiation

Hypothalamic NPC from 1 Control (Inline graphic) and LBW (Inline graphic) newborn males were cultured in differentiating media. NPC were harvested and protein expression of neuronal marker (Tuj1)) and astrocyte marker (GFAP) were determined. Values are fold change (mean ± SE); * P < 0.05 LBW vs. Control.