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. Author manuscript; available in PMC: 2012 Oct 16.
Published in final edited form as: Brain Res. 2011 Jan 6;1378:29–42. doi: 10.1016/j.brainres.2010.12.080

Figure 3.

Figure 3

Figure 3

Figure 3

A: Evidence for NPC and Neuronal Leptin Receptor

NPC grown in complete or differentiating media were immunostained for leptin receptor (ObRb), neuronal marker (Tuj1) and nuclear stain (DAPI).

B: Leptin and Insulin Stimulated Hypothalamic NPC Proliferation

Hypothalamic NPC from 1 day old Control (Inline graphic) and LBW (Inline graphic) males were cultured in complete media. On second day of seeding, NS were treated with leptin/insulin every 48h for 8 days and cell proliferation rate was determined. Values are percentage of untreated Control (mean ± SE); * P < 0.05 vs. untreated NS; “C” P < 0.05 LBW vs. Control.

C: Leptin and Insulin Stimulated Hypothalamic Nestin Expression

Hypothalamic NS from 1 day old Control (Inline graphic) and LBW (Inline graphic) males were cultured in complete media. On second day of seeding, NS were treated with leptin/insulin every 48h for 8 days. Nestin protein expression (marker of NPC) was determined. Values are mean ± SE; * P < 0.05 vs. untreated NS; “C” P < 0.05 LBW vs. Control.