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Spore-display of EGFP. (A) Flow cytometry analysis. Black line, BT spores harboring pSD1 as a negative control; green line, BT spores harboring pSD-EGFP; (B) Fluorescence assay. 1, BT spores harboring pSD1 as a negative control; 2, BT spores harboring pSD-EGFP; (C) Confocal microscopy analysis. The inset shows an optical microscopic image. Scale bars represent 5 μm.
