Table 1. Clinicopathological features of the primary RB tumors following the International Intraocular Retinoblastoma Classification (IIRC) with gene expression (by qRT–PCR).

S/N	Age/Sex	Clinico pathological features	Log 2 ratio fold change
			ELK1	GTSE1	CDK6	E2F4	DRAM	CDH1	SNAI1
1	4/M	OS:PD tumor seen in iris surface, trabecular meshwork, diffuse CI with measuring >3 mm thickness, prelaminar, laminar invasion, invasion of anterior & middle portion of sclera .	++	-	++	++	-	NS	+
2	3/F	OS: WD with formation of fleurettes, prelaminar invasion of ON.	++	++	++	++	+	NS	++
3	2/M	OS:WD massive CI >3 mm, tumor cells invading the anterior, middle and posterior border of sclera with spill over into the orbital tissue.	++	++	++	++	+	+	NS
4	4/M	OD:PD, there is full thickness diffuse CI measuring >3 mm, tumor cells touching the anterior border of scelra, prelaminar invasion of ON	++	++	++	++	+	NS	++
5	8/ F	OD:UD, tumor cells adherent to iris surface, invasion of ciliary process, diffuse full thickness CI >3 mm, invasion of prelaminar, laminar & post laminar portion of ON,outer margin of sclera.	++	++	++	++	+	NS	++
6	6mon/F	OS;MD, CI measuring >3mm, prelaminar invasion and laminar invasion of the ON.	++	++	++	++	+	+	++
7	3/M	OS:PD, focal CI measuring <3 mm.	++	++	++	++	+	NS	++
8	5/M	OD:UD prelaminar invasion of ON	++	-	++	++	+	NS	++
9	2/F	OD: PD, tumor cells in iris surface, full thickness CI measuring 10 mmx7 mm.Sclera invasion.	++	++	++	++	+	NS	++
10	1/F	OS: WD, focal choroid invasion measuring <3mm.	++	++	++	++	+	NS	++

F:female; M:male; MD:moderately differentiated; PD:poorly differentiated; WD:well differentiated; OD:right eye; OS:left eye; CI:choroidal invasion; ON:optic nerve; ++: Up-Regulated genes; +: Down-Regulated; -: Negative; NS: Not significant fold change