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. 2012 Oct 3;18:2420–2437.

Table 2. The primer sequences used for validation of the key genes in the primary retinoblastoma tumors by qRT–PCR.

Gene name Sense/Antisense: Sequences
ETS oncogene family (ELK1)
FP: 5′GAAGAATCACACCCTTGGAA3′
 
RP: 5′GACAAAGGAATGGCTTCTCA 3′
G-2 and S-Phase expressed1(GTSE1)
FP: 5′ACGTGAACATGGATGACCCTA3′
 
RP: 3′GTTCGGGAACCGGATTATTTA5′
cyclin dependent kinase 6 (CDK6)
FP: 5′CTGAATGCTCTTGCTCCTTT3′
 
RP: 5′AAAGTTTTGGTGGTCCTTGA3′
E2Ftranscriptionfactor4,p107/p130binding(E2F4)
FP: 5′GGGCAGAAGAAGTACCAGATTCA3′
 
RP: 5′GCTCCATGCCTCCTTGTTCA3′
v – crk sarcoma virus CT10 oncogene homolog(avian; CRK) transcript variant
FP: 5′CCGGGACAAGCCTGAAGAGC3′
 
RP: 5′GGCCCACCCAGTGGCTGTGG3′
CDH1
FP: 5′TCGACACCCGATTCAAAGTGG3′
 
RP: 5′TTCCAGAAACGGAGGCCTGAT3′
SNAIL homolog 1 (Drosophila; SNAI1)
FP: 5′TATGCTGCCTTCCCAGGCTTG3′
 
RP: 5′ATGTGCATCTTGAGGGCACCC3′
MMP 2
FP: 5′AGATCTTCTTCTTCAAGGACCGGTT3′
 
RP: 5′GGCTGGTCAGTGGCTTGGGGTA3′
MMP 9
FP:5′GCGGAGATTGGGAACCAGCTGTA3′
  RP: 5′GACGCGCCTGTGTACACCCACA3′