Skip to main content
. Author manuscript; available in PMC: 2013 Jan 23.
Published in final edited form as: Angew Chem Int Ed Engl. 2011 Dec 16;51(4):993–999. doi: 10.1002/anie.201106060

Figure 1.

Figure 1

Kinetically controlled ligation of peptide segments Ala1-Cys(Acm)29-αCOSC6H4CH2COOH and Cys30-Leu67-α COSCH2CH2OH. A) t < 1 min. B) t = 1 h. C) After adding MESNa for 2 h. D) After HPLC purification. ESI-MS (inset) gave an observed mass of 8090.0 ± 0.2 Da (calcd. 8089.1 Da, average isotopes). Analytical HPLC traces (λ = 214 nm) are shown.