Table 1. Measuring the effects of BART miRNAs on expression levels of cellular proteins using 2D DIGE.
Spot | Protein | NCBI AccessionNumber | Gene | Fold change of protein (BJAB BARTs/BJAB Empty) |
1 | Heat shock 70 kDa protein 5 | GI: 16507237 | HSPA5 | −1.31 |
2 | Heat shock 90 kDa protein 1, beta | GI: 20149594 | HSP90AB1 | −1.45 |
3 | Heat shock 70 kDa protein 9 precursor | GI: 24234688 | HSPA9 | −1.38 |
5;6 | Gelsolin isoform b | GI: 38044288 | GSN | −1.48; −1.40 |
6;7 | Ezrin | GI: 46249758 | EZR | −1.60; −1.31 |
8 | Synaptotagmin binding, cytoplasmic RNA interacting protein,isoform CRA_e | GI: 119569012 | SYNCRIP | −1.33 |
9 | Calreticulin precursor variant | GI: 62897681 | CALR | −1.65 |
10 | Heat shock protein 60 | GI: 77702086 | HSPD1 | −1.47 |
11 | Protein disulfide-isomerase A3 precursor | GI: 21361657 | PDIA3 | −1.62 |
12 | T-complex protein1 isoform a | GI: 57863257 | TCP1 | −1.33 |
13 | Nucleosome assembly protein 1-like 1 | GI: 4758756 | NAP1L1 | −2.00 |
14; 15; 16 | ACTB protein | GI: 15277503 | ACTB | −1.44; −2.14; −1.87 |
17 | Annexin VII isoform 1 | GI: 4502111 | ANXA7 | −1.49 |
18 | Alpha-enolase isoform 1 | GI: 4503571 | ENO1 | −1.40 |
19 | SET | GI: 145843637 | SET1 | −1.89 |
20 | Nudix (nucleoside diphosphate linked moiety X)-type motif 5,isoform CRA_a* | GI: 119606726 | NUDT5 | −1.31 |
21 | Serine/arginine-rich splicing factor 1 isoform 1 | GI: 5902076 | SFRS1 | −1.37 |
22 | Inorganic pyrophosphatase | GI: 11056044 | PPA1 | −1.36 |
23 | Bisphosphoglycerate mutase | GI: 4502445 | BPGM | −1.32 |
24 | Phosphoglycerate mutase 1 (brain) | GI: 38566176 | PGAM1 | −1.51 |
25 | Glutathione S-transferase | GI: 2204207 | GSTP1 | −1.42 |
26 | Peroxiredoxin-1 | GI: 4505591 | PRDX1 | −2.03 |
27; 28 | Cellular nucleic acid-binding proteinisoform 1 | GI: 187608726 | CNBP1 | −1.35; −1.32 |
29 | Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrialisoform 2 | GI: 4503423 | DUT | −1.30 |
30 | Hypothetical protein LOC51237* | GI: 117938314 | MZB1 | −1.57 |
31 | Eukaryotic translation initiation factor 5A-1 isoform B | GI: 4503545 | EIF5A | −2.10 |
32 | Cofilin-1 | GI: 5031635 | CFL1 | −1.35 |
33 | 60S acidic ribosomal protein P2 | GI: 4506671 | RPLP2 | −2.08 |
34 | Fatty acid-binding protein, epidermal | GI: 4557581 | FABP5 | −1.49 |
35 | ISG15 ubiquitin-like modifier | GI: 14550514 | ISG15 | −1.48 |
Not characterized as a protein.
Full list of candidates identified as being regulated by the BART miRNAs via 2D DIGE, along with changes in the protein levels observed in the presence of the miRNAs is shown.