Table 3. Immunoprecipitating RISCs from BJAB cells that express BART miRNAs constitutively.

		Fold Enrichment of miRNAs in RISCs*
miRNA	Cell	Anti-GST	Anti-Ago2
miR-150	BJAB Empty	0.2	536
	BJAB BARTs	0.2	1052
BART10	BJAB Empty	1.3	2.1
	BJAB BARTs	2.1	869

^*Fold enrichment of miRNAs was normalized to the amount of miRNAs in input.

Expression levels of cellular miR-150 and EBV’s miRBART10 were measured by stem-loop RT-PCR in BJAB BARTs and BJAB Empty RNAs immunoprecipitated with either anti-Ago2 or anti-GST antibody. Fold enrichment in each experiment was normalized to the Input sample. miR-150 is enriched in α-Ago2 immunoprecipitates in both BJAB BARTs and BJAB Empty cell lines, whereas BART10 is only enriched in immunoprecipitates of BJAB BARTs cells, as anticipated.