Table 6. Evaluating the utility of criteria used to reduce the number of candidate mRNAs.
| Gene | Fold enrichment of mRNAsin RISCsa | Expression level ofencoded proteinb | Predicted miRBARTs binding siteson the 3′UTR |
| ACTB | 1.6 | −1.4 | None |
| ANXA7 | 1.5 | −1.5 | None |
| CALR | 1.8 | −1.7 | None |
| CFL1 | 1.8 | −1.4 | 6-3p, 19-3p |
| EIF5A | 1.7 | −2.1 | 6-3p |
| ENO1 | 2.3 | −1.4 | None |
| GSTP1 | 1.9 | −1.4 | None |
| PRDX1 | 2.0 | 2.0 | None |
| RPLP2 | 1.8 | −2.1 | 19-3p |
a
Fold enrichment of mRNAs in RISCs of BJAB BARTs cells relative to that of BJAB Empty cells were analyzed by deep sequencing.
b
Expression level of proteins in BJAB BARTs cells relative to that in BJAB Empty cells were detected by 2D DIGE.
The genes identified as potential candidates for being regulated by BART miRNAs using both approaches are shown. Only 3 of these transcripts had predicted miRNA binding sites, however, these miRNAs were expressed inefficiently. None warranted selection for validation.